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KMID : 0613820170270101097
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Journal of Life Science
2017 Volume.27 No. 10 p.1097 ~ p.1103
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Induction of Sexual Maturation in Female Eels (Anguilla japonica) Using an Osmotic Pump
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Kim Jung-Hyun
Kim Dae-Geun
Kim Hyo-Won
Lee Bae-Ik
Kim Shin-Kwon
Jun Je-Cheon
Myeong Jeong-In
Kim Dae-Jung
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Abstract
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For the artificial induction of the sexual maturation of Anguilla japonica, salmon pituitary extract (SPE) is continuously injected into females, and the eggs obtained from artificial sexual maturation are artificially fertilized with sperms and hatched. However, repeated injection of SPE in the abdominal cavity causes tremendous stress in females, which may prevent their complete sexual maturation and reduce the immune system function, ultimately resulting in death. In addition, the poor quality of the ovulated eggs can reduce the hatching and survival rate of larvae. In the present study, sexual maturation of females was induced by inserting an osmotic (OS) pump containing hormone analogs known to effectively induce sexual maturation into the abdominal cavity of female eels, and the effect of the OS pump on the induced sexual maturation was investigated. Our study results showed that the gonadosomatic index (GSI) was significantly higher in the fish subjected to SPE injection than those subjected to human chorionic gonadotropin (hCG), gonadotropin-releasing hormone analog (GnRHa), and methyl testosterone (MT) injections, either separately or in combination. In addition, a histological analysis showed that the oocytes in the SPE OS pump groups were more mature (entered the nuclear shift stage) than those in the other groups. These results suggest that an osmotic pump containing hormone analogs can be used to induce sexual maturation in female A. japonica artificially.
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KEYWORD
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Eel, gonadal development, osmotic pump, reproduction
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